Multiplex SNP discrimination.

نویسندگان

  • Daniel J Fish
  • M Todd Horne
  • Robert P Searles
  • Greg P Brewood
  • Albert S Benight
چکیده

Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.

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عنوان ژورنال:
  • Biophysical journal

دوره 92 10  شماره 

صفحات  -

تاریخ انتشار 2007